Organic Intermediate Isopropyl-Beta-D-Thiogalactopyranoside/Iptg CAS 367-93-1

• CAS No.: 367-93-1
• Formula: C9h18o5s
• EINECS: 206-703-0
• Type: Pharmaceutical Intermediates
• Appearance: Powder
• Quality: High Purity
• Model NO.: 367-93-1
• Colour: White
• Custom Clearence: 100% Safe Custom Clearence
• Lead Time: 1-3 Days
• Express Time: 3-7 Days
• Payment Method: T/T, Western Union, L/C, etc.
• Transport Package: Packed by Aluminum Foil Bag or Cardbaord Drum
• Specification: 99.9% Purity White Powder
• Trademark: Harvest
• Origin: Wuhan, Hubei, China
• Production Capacity: 50000kg/Year

Product Description

Product Description

Product Name	IPTG (Isopropyl β-D-thiogalactoside)
CAS No.	367-93-1
Molecular Formula	C9H18O5S
Molecular Weight	238.3
Grade Standard	Medicine Grade
Purity	99% min

.
 

Isopropyl β-D 1thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG, unlike allolactose, is not hydrolyzable by βgalactosidase, its concentration, therefore, remains constant in an experiment. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, the different final concentration of IPTG may also be used.
 

Function
 

IPTG is often used for induction β- Cloning experiment of galactosidase activity. It is often used in combination with X-gal or bluo gal for blue-white screening of recombinant bacterial colonies. These colonies can be chemically book to induce the expression of lac operon in E. coli. IPTG binds to lacI repressor protein and changes its conformation to prevent β- Inhibition of galactosidase coding gene lacZ.

 

 

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